Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Elucidating the cellular mechanism for E2-induced dermal fibrosis

Fig. 3

E2-induced EGR1 transcription and translation and its effects on TGFβ1 and TGFβ2 transcription in vitro. a Steady-state transcript levels of EGR1 6 h post vehicle vs. E2 stimulation in vitro. b Representative immunoblot of EGR1 protein 16 h post  E2 stimulation. c Densitometry of EGR1 immunoblot. df Steady-state transcript levels of EGR1 (d), TGFβ1 (e), and TGFβ2 (f) in primary human dermal fibroblasts transfected with siRNA against EGR1 24 h post E2 stimulation. Control, CTL. Normalized to B2M (a, df) or Tata-binding protein (TBP) (c). Bars = mean ± SEM. Data from af includes ≥ 6 independent experiments using dermal fibroblasts from ≥ 6 different donors. Statistical tests: Wilcoxon matched-pairs signed rank test (a, c) and parametric, one-way ANOVA with Sidak’s multiple comparisons post hoc test (d, f), and non-parametric one-way ANOVA using Dunn’s multiple comparisons (e). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005

Back to article page