Fig. 3From: Elucidating the cellular mechanism for E2-induced dermal fibrosisE2-induced EGR1 transcription and translation and its effects on TGFβ1 and TGFβ2 transcription in vitro. a Steady-state transcript levels of EGR1 6 h post vehicle vs. E2 stimulation in vitro. b Representative immunoblot of EGR1 protein 16 h post E2 stimulation. c Densitometry of EGR1 immunoblot. d–f Steady-state transcript levels of EGR1 (d), TGFβ1 (e), and TGFβ2 (f) in primary human dermal fibroblasts transfected with siRNA against EGR1 24 h post E2 stimulation. Control, CTL. Normalized to B2M (a, d–f) or Tata-binding protein (TBP) (c). Bars = mean ± SEM. Data from a–f includes ≥ 6 independent experiments using dermal fibroblasts from ≥ 6 different donors. Statistical tests: Wilcoxon matched-pairs signed rank test (a, c) and parametric, one-way ANOVA with Sidak’s multiple comparisons post hoc test (d, f), and non-parametric one-way ANOVA using Dunn’s multiple comparisons (e). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005Back to article page