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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-β/SMAD2 signaling

Fig. 4

USP15 can form a complex with ERK2. a Rat articular chondrocytes were co-immunoprecipitated and examined with the indicated antibodies. Co-immunoprecipitation with anti-USP15 antibodies and immunoblotting with anti-USP15 or ERK2 antibodies. IgG immunoprecipitation was a negative control. b Immunofluorescent detection of Flag-USP15, Flag-USP15C269S, and HA-ERK2 in ATDC5 cells. Nuclei was counterstained by DAPI (scale bars = 50 μm). c 293T cells were co-transfected with the indicated plasmids, and cell lysates were co-immunoprecipitated with the indicated antibodies and immunoblotted with anti-Flag or anti-HA antibody

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Arthritis Research & Therapy

ISSN: 1478-6362

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