|
Parameter
|
Bivariate (r)
|
Multivariate (β, r2)
|
|---|
|
% Adipose
|
Timepoint
|
Diet (β)
|
% Adipose (β)
|
Timepoint (β)
|
Whole model (r2)
|
|---|
|
Experimental (Obesogenic)
|
Western
|
|---|
|
Behavioral
|
|
Grip force
|
− 0.41***
|
− 0.45***
|
− 21.96**
|
− 6.23
|
0.4
|
− 0.841**
|
0.45***
|
|
Mechanical sensitivity (Von Frey)
|
− 0.42***
|
− 0.21*
|
− 0.35
|
− 0.14
|
− 0.0007
|
− 0.006
|
0.26***
|
|
Cold sensitivity (Acetone)
|
0.04
|
0.01
|
–
|
–
|
–
|
–
|
0.08
|
|
Tail suspension
|
|
Rearing
|
0.01
|
0.11
|
–
|
–
|
–
|
–
|
0.02
|
|
Immobility
|
0.18
|
0.19
|
–
|
–
|
–
|
–
|
0.05
|
|
Self-support
|
− 0.22*
|
− 0.30**
|
1.73
|
− 0.46
|
− 0.71
|
− 0.74*
|
0.10*
|
|
Stretch
|
0.06
|
0.10
|
–
|
–
|
–
|
–
|
0.07
|
|
Open field
|
|
Locomotion
|
− 0.49***
|
− 0.35***
|
− 1226
|
− 59.3
|
− 92.7
|
− 93.5***
|
0.33***
|
|
Rearing
|
− 0.52***
|
− 0.40***
|
− 0.88
|
− 7.41
|
− 2.87*
|
− 2.059***
|
0.39***
|
|
Histopathology
|
|
IVD degeneration (average Boos)
|
0.065
|
0.04
|
–
|
–
|
–
|
–
|
0.03
|
|
Knee OA (cumulative OARSI)
|
0.07
|
0.08
|
–
|
–
|
–
|
–
|
0.05
|
|
Synovitis
|
0.68***
|
0.102
|
3.535
|
− 0.692
|
0.325*
|
0.063
|
0.478***
|
|
Neuro-inflammation
|
|
Lower spinal cord
|
|
GFAP
|
0.23
|
–
| | | |
–
|
0.30
|
|
IBA-1
|
0.23
|
–
|
–
|
–
|
–
|
–
|
0.06
|
|
CGRP
|
0.024
|
–
|
–
|
–
|
–
|
–
|
0.18
|
|
Upper spinal cord
|
|
GFAP
|
0.21
|
–
|
–
|
–
|
–
|
–
|
0.08
|
|
IBA-1
|
0.33
|
–
|
–
|
–
|
–
|
–
|
0.12
|
|
CGRP
|
0.31
|
–
|
–
|
–
|
–
|
–
|
0.18
|
|
Systemic factors
|
|
IL-1B
|
0.04
|
0.21
|
–
|
–
|
–
|
–
|
0.12
|
|
IL-6
|
0.06
|
0.22
|
–
|
–
|
–
|
–
|
0.09
|
|
IL-10
|
0.03
|
0.21
|
–
|
–
|
–
|
–
|
0.14
|
|
IP-10
|
0.03
|
0.07
|
–
|
–
|
–
|
–
|
0.001
|
|
KC
|
0.002
|
0.25
|
–
|
–
|
–
|
–
|
0.08
|
|
MCP-1
|
0.23
|
0.04
|
–
|
–
|
–
|
–
|
0.18
|
|
TNFa
|
0.03
|
0.30*
|
–
|
–
|
–
|
–
|
0.16
|
|
VEGF
|
0.01
|
0.12
|
–
|
–
|
–
|
–
|
0.06
|
- Experimental/obesogenic diets include the high-fat and western diet
- *P < 0.05, **P < 0.01, ***P < 0.001
