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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Does methotrexate influence COVID-19 infection? Case series and mechanistic data

Fig. 2

MTX down-regulates ACE2 expression. a Real-time PCR showing quantification of mRNA for ACE2 in the various segments off the intestine and the lung in mice treated with methotrexate (MTX; N = 3 per group per experiment; data summarized from two independent experiments). b Representative immune histochemistry staining for ACE2 (brown color, arrowheads) in the intestine and the lung of mice treated with methotrexate or vehicle for 3 days. Isotype control antibody staining is also shown. c Quantification of ACE2 expression in immune histochemistry (N = 3 per group per experiment; data summarized from two independent experiments). d Representative immunoblot for ACE2 and actin (control) in intestinal organoids treated with two doses of methotrexate including densitometric quantification

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