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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Stimulation of regulatory T cells with Lactococcus lactis expressing enterotoxigenic E. coli colonization factor antigen 1 retains salivary flow in a genetic model of Sjögren’s syndrome

Fig. 3

LL-CFA/I treatment augments IL-10 production and diminishes IFN-γ and IL-17. MLN, splenic, and HNLN lymphocytes were stimulated with anti-CD3 plus anti-CD28 monoclonal antibodies (mAbs) for 4 days. Collected culture supernatants were analyzed for production of a IFN-γ, b IL-17, and c IL-10. Depicted are the means ± SEM of duplicate cultures from individual mice; *P < 0.05, **P < 0.01 for LL-CFA/I versus LL vector or PBS groups. d mRNA analysis of Th1, Th17, and Treg cells subsets was conducted. RNA extracted from 2-day anti-CD3 plus anti-CD28 mAb-stimulated MLN lymphocytes from PBS-, medium dose LL vector-, and low-dose LL-CFA/I-treated groups (4 mice/group) were analyzed by QRT-PCR for Th1: T-bet, IFN-γ, and TNFα; Th17: Rorγt and IL-17; and Tregs: Foxp3, TGF-β, and IL-10 mRNA expression. Fold changes versus expression obtained by lymphocytes from the PBS group are depicted. Significance was determined using a unpaired t test for comparisons: *P < 0.05, **P < 0.01 LL CFA/I compared to LL vector-treated group

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Arthritis Research & Therapy

ISSN: 1478-6362

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