Skip to main content
Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Stimulation of regulatory T cells with Lactococcus lactis expressing enterotoxigenic E. coli colonization factor antigen 1 retains salivary flow in a genetic model of Sjögren’s syndrome

Fig. 6

Adoptive transfer of total CD4+ T cells from LL-CFA/I-treated SjS mice restores SFR and increases both the frequency and activity of Tregs in recipient diseased SjS mice. a The donor pool composed of SjS females (8/group; 16 wks of age) treated four times at 4 wk intervals with either LL vector or LL-CFA/I similar to that described above. Two wks following the last dose given on wk 28, total LN and splenic CD4+ T cells were purified to > 95% purity, and 1 × 106 CD4+ T cells/mouse were i.v. injected into recipient SjS females (> 20 wks of age) (10/group). The percentage of CD25+CD4+ T cells in each group was measured 2 wks later. b Only recipients given CD4+ T cells from LL-CFA/I-treated mice showed restored SFR; ***P < 0.0001, ****P < 0.00001 versus LL vector-treated mice. MLN Treg composition as percent CD4+ T cells in the recipients are shown in representative FACS plots and histograms: c Foxp3+, d IL-10+, and e TGF-β+. fh Frequency of CD25+CD4+ T cell subsets as f CD25+CD4+, g Foxp3+ CD25+CD4+, h IL-10+ CD25+CD4+, and i TGF-β+ CD25+CD4+ T cells in recipients are depicted; *P < 0.05, **P < 0.01 for comparison between recipients given donor CD4+ T cells from LL-CFA/I or LL vector. Data are representative of two experiments

Back to article page