Fig. 5

Descriptive RNA-seq data and real-time PCR. a Principal component analysis of chondrocytes samples from wt (n = 3) and Postn^−/− (n = 3) mice showed distinct grouping based on gene expression differences. b MA (log₂ ratio to mean expression) plot depicts expression fold change and averaged expression level of differentially expressed transcripts. c Volcano plot of all genes expressed greater than 1 count-per-million in all 6 samples where the observed log₂ fold change is on the x-axis and the unadjusted P value converted to the −log₁₀ scale is on the y-axis. Twelve genes with unadjusted P ≤ 0.05 are highlighted in red when log₂ fold changes > + 2 and blue when < – 2. d Real-time PCR performed on chondrocytes isolated from an independent cohort of wt (n = 6) and Postn^−/− (n = 6) mice revealed an agreement with the RNA-seq data. (Mann Whitney test)