Table 2 Sequence and characteristics of primers used for real-time PCR
From: Periostin loss-of-function protects mice from post-traumatic and age-related osteoarthritis
Gene symbol | Forward primer (5′ → 3′) | Location | Reverse primer (5′ → 3′) | Location | Amplicon size | Accession no. |
|---|---|---|---|---|---|---|
Dscaml1 | aggctgaagaggctacgaga | 4972–4991 | gaggtcctttcacaggggtg | 5062–5043 | 91 bp | NM_001081270.2 |
Tm4sf1 | actgggtttggcagaaggac | 1006–1025 | tgggctcatagcacttggac | 1127–1108 | 122 bp | NM_008536.4 |
Ndufs5 | acagccctataagaacgccg | 148–167 | tgtaccgaagcaagcactct | 287–268 | 140 bp | NM_001030274.1 |
Srsf10 | acgtcgggaatttggtcgtt | 234–253 | agcgtcttcagcatcacgaa | 357–338 | 124 bp | NM_010178.3 |
Gapdh | aggtcggtgtgaacggatttg | 100–120 | tgtagaccatgtagttgaggtca | 222–200 | 123 bp | NM_001289726.1 |