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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Upregulated endonuclease Regnase-1 suppresses osteoarthritis by forming a negative feedback loop of catabolic signaling in chondrocytes

Fig. 2

Overexpression of Regnase-1 in joint tissue ameliorates post-traumatic OA cartilage destruction in mice. a Representative images of Safranin-O staining (left) and Regnase-1 immunostaining (right) of WT mice IA-injected with empty virus (Ad-C; n = 5 mice) or Ad-Regnase-1 (n = 8 mice). b Representative RT-PCR images of the indicated molecules in chondrocytes treated with the indicated MOI of Ad-C or Ad-Regnase-1 (n = 5). ce Sham- or DMM-operated WT mice were IA-injected with Ad-Control (Ad-C) or Ad-Regnase-1 (Ad-R). Representative immunostaining images of Regnase-1 and MMP3 in cartilage sections (c), Safranin-O staining images (d), and scoring of OARSI grade, osteophyte size, and subchondral bone plate (SBP) thickness (e) at 8 weeks after operations (n = 15 mice per group). Vales in e are means ±95% CI with Mann–Whitney U test for OARSI grade and means ± s.e.m. with two-tailed t test for osteophyte size and SBP thickness. **p < 0.005. ns, not significant. Scale bar: 50 μm

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