Fig. 3

Knockdown or genetic ablation of Regnase-1 exacerbates OA pathogenesis in mice. a, b Sham- or DMM-operated WT mice were IA-injected with Ad-shScramble (Ad-shC) or Ad-shRegnase-1 (Ad-shR) and sacrificed at 6 weeks after operation (n = 8 mice per group). Safranin-O staining images (a) and scoring of OARSI grade, osteophyte size, and SBP thickness (b). c, d WT (Zc3h12a^+/+) and Zc3h12a^+/− mice were subjected to sham operation or DMM surgery and sacrificed at 6 weeks after operation (n = 15 mice per group). Representative Safranin-O staining images (c) and scoring of OARSI grade, osteophyte size, and SBP thickness (d). Values in b and d are means ±95% CI with Mann–Whitney U test for OARSI grade and means ± s.e.m. with two-tailed t test for osteophyte size and SBP thickness. *p < 0.05 and **p < 0.005. ns, not significant. Scale bar: 50 μm