Fig. 4

Effects of Regnase-1 overexpression on the expression of matrix-degrading enzymes and ECM molecules. a–c Primary-culture chondrocytes were infected with 800 MOI of Ad-C or Ad-Regnase-1 (Ad-R) for 12 h, and exposed to IL-1β (0.01 ng/ml) for an additional 24 h. mRNA levels of the indicated molecules were quantified by qRT-PCR analysis (a, b, n = 6). d–f Primary-culture chondrocytes were pre-infected with 400 MOI of Ad-C or Ad-Regnase-1 (Ad-R) for 12 h and additionally infected with 400 MOI of Ad-HIF-2α for 24 h. Untreated chondrocytes were infected with 800 MOI of Ad-C as a control. The indicated molecules were quantified by qRT-PCR analysis (d, e, n = 6). Representative Western blot images of the indicated molecules are presented in c (n = 6). Means ± s.e.m. with one-way ANOVA with Bonferroni’s post hoc test. *p < 0.05 and ***p < 0.0005. ns, not significant