Fig. 5

Zc3h12a^−/− chondrocytes exhibit enhanced expression of matrix-degrading enzymes when treated with IL-1β or Ad-HIF-2α. a, b WT and KO (Zc3h12a^−/−) chondrocytes were treated with 0.01 ng/ml of IL-1β for 36 h mRNA levels (a) and protein levels (b) of the indicated molecules were determined by qRT-PCR analysis and Western blotting, respectively (n = 6). c, d WT and KO (Zc3h12a^−/−) chondrocytes were infected with 800 MOI of Ad-HIF-2α for 36 h. mRNA levels (c) and protein levels (d) of the indicated molecules were determined by qRT-PCR analysis and Western blotting, respectively (n = 6). Values are means ± s.e.m. with one-way ANOVA with Bonferroni’s post hoc test. *p < 0.05, **p < 0.005, ***p < 0.0005. ns, not significant