Fig. 6

SAA3 is a target of Regnase-1 in chondrocyte. a, b Number of genes modulated by Regnase-1 (a) and list of the top 13 genes that were most markedly regulated by Ad-shRegnase-1 (b). c Luciferase assay (n = 4) in chondrocytes transfected with a reporter plasmid containing the SAA3 3′-UTR sequence. Chondrocytes were co-transfected with empty vector (EV), WT Regnase-1 (WT), or D141N Regnase-1 (D141N). d qRT-PCR analysis (n = 6) of SAA family members in chondrocytes infected with Ad-Regnase-1 or Ad-shRegnase-1 for 36 h. e Quantitation of secreted SAAs by ELISA in chondrocytes treated with IL-1β (0.1 ng/ml, 36 h) or infected with the indicated MOI of Ad-C or Ad-SAA3 (36 h) (n = 4). Means ± s.e.m. and one-way ANOVA with Bonferroni’s post hoc test. *p < 0.05, **p < 0.005, ***p < 0.0005