Fig. 7

Representative confocal images of the right knee of a Ccr2^RFP mouse 8 weeks after DMM surgery, immunostained with CGRP. a CGRP/CCR2 overlay of the lateral compartment of the knee; b magnified image of a showing CCR2, CGRP signals and the overlay of both images. White arrows indicate an example of overlap between CGRP and CCR2 signals in the lateral synovium (CCR2 receptors present on CGRP+ nerve fibers). c CGRP/CCR2 overlay of the medial compartment of the knee; d, e magnified of c showing CCR2, CGRP signals and the overlays of both images. c, d White arrows indicate an example of an overlap between CGRP and CCR2 signal in the medial synovium (CCR2 receptors present on CGRP+ nerve fibers). c, e Blue arrows point to a CGRP/CCR2-positive channel in the medial subchondral bone. f, g Quantification of the CGRP+ and CCR2+/CGRP+ channels in the subchondral bone of naïve and DMM knees. Unpaired two-tailed t test. Scale bar for a, c, d = 100 μm. Scale bar for b, e = 25 μm. **p < 0.01. Mean ± SEM