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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Regulation of osteoclastogenesis by mast cell in rheumatoid arthritis

Fig. 2

Effect of IL-33 on the expression of tryptase and chymase in mast cells. a Human mast cells were culture with 100 ng/ml of IL-33 for 24 h, after which c-kit+ FcεRI+ cells were gated for further analysis. b Percentages of tryptase- and chymase-positive mast cells were measured by flow cytometry. c Gene expression levels of tryptase and chymase were measured using real-time PCR. Data were normalized to the expression level of beta-actin and reported in relative expression units. Data are presented as mean ± SEM from four independent experiments. *p < 0.05 and **p < 0.01

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