Fig. 1
Distinct metabolic features of NP and AF cells in cell culture. Rabbit NP and AF cells were grown on monolayer cultures in Gibco Ham’s F12 media at 5% O2, and the concentrations of glucose and lactate in their culture media were measured by Accutrend Strips at different time points. NP cells in cell culture are highly glycolytic that readily consumed glucose (a) and produced and secreted an abundance of amount of lactate (b) in a time-dependent manner. Under the same culture condition, AF cells both consumed glucose (c) and produced lactate (d) at much slower rates compared to NP cells, suggesting that AF cells are less glycolytic and more aerobic in their metabolism