Fig. 6
From: Cold-inducible RNA-binding protein (CIRP) potentiates uric acid-induced IL-1β production
a Effects of MCC950 on MSU-induced IL-1β synthesis from CIRP-primed neutrophils. CIRP (300 ng/ml, for 6 h)-primed neutrophils were pretreated with or without MCC950 for 30 min, and then stimulated with MSU (200 μg/mL) for 18 h. Supernatants were analyzed for IL-1β production by ELISA. Values represent the mean ± SD of two independent experiments. Significant difference was observed at a threshold of p < 0.01 between CIRP/MSU-stimulated neutrophils with and without pretreatment of MCC950 (10− 6 M). b Effects of MCC950 on MSU-induced cleaved-IL-1β (p17) expression of CIRP-primed neutrophils. CIRP (300 ng/ml, for 6 h)-primed neutrophils were pretreated with or without MCC950 for 30 min, and then stimulated with MSU (200 μg/mL) for 18 h. Cellular lysates were analyzed by western blotting using anti-cleaved-IL-1β antibodies. Two experiments were performed using different neutrophils, and a representative result is shown