Fig. 7From: Cold-inducible RNA-binding protein (CIRP) potentiates uric acid-induced IL-1β productionCIRP-induced NLRP3 expression in neutrophils. CIRP (300 ng/ml, for 30 min)-primed neutrophils were pretreated with or without MCC950) for 30 min and stimulated with MSU (200 μg/mL) for 18 h. Cellular lysates were analyzed by western blotting using anti-NLRP3 antibodies. β-actin was the loading control. Two experiments were performed using different neutrophils, and a representative result is shownBack to article page