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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Fargesin ameliorates osteoarthritis via macrophage reprogramming by downregulating MAPK and NF-κB pathways

Fig. 5

Fargesin protects chondrocytes from catabolism via paracrine macrophage mechanism. a–f Immunostaining and quantitative analysis of cells positive for MMP13, RUNX2, and Col X in CIOA mice treated with vehicle or fargesin 3 and 6 weeks after intra-articular injection of collagenase (n = 4). Scale bar, 50 μm. Higher magnification is shown on the top right. Scale bar, 100 μm. g, h Raw264.7 cells were treated with vehicle (served as Con) or fargesin (served as Far) for 24 h and conditioned medium (CM) were collected. LPS-induced Raw264.7 cells were co-treated with vehicle (served as M1+Vehicle) or fargesin (served as M1+Far) for 24 h and CM were collected. Quantitative PCR analysis of MMP13, RUNX2, Col X, Col2, and Sox9 in ATDC5 cells treated with different CM from Raw 264.7 cells (respectively Con, Far, M1+vehicle, and M1+Far) for 24 h. i ATDC5 cells were treated with vehicle (served as Con) for 24 h and CM were collected. IL-1β-induced ATDC5 cells were co-treated with vehicle (served as IL-1β-CM) or fargesin (served as IL-1β-CM+Far) for 24 h and CM were collected. Western blot results for p-p65, p65, p-ERK, ERK, p-p38, and p38 in Raw264.7 cells treated with different CM (respectively Con, IL-1β-CM, and IL-1β-CM+Far) from ATDC5 (n = 3). AC, articular cartilage; MM, medial meniscus

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