Fig. 2

IL-1β production by THP-1 cells after crystal stimulation. a Release of IL-1β by THP-1 cells cultured during 6 h with crystals (1mg/ml) in media with or without 10% fetal bovine serum (FBS) (n = 3). b Kinetic of IL-1β production by THP-1 cells cultured with a patient’s calcification (CAL, 1mg/ml) and synthetic hydroxyapatite (sHA, 1 mg/ml) or without crystal (CT) (n = 3). c IL-1β release in cultures containing only crystals compared to THP-1 cells after crystal stimulation (N = 3 patients). d IL-1β quantification in supernatants of THP-1 after 6 h of crystal stimulation with increasing concentrations of calcifications of 9 different patients (N = 9). sHA, synthetic hydroxyapatite; CAL, human calcification; CT, control condition without crystals. *p ≤ 0.05