Fig. 5

In vivo effects of human calcifications in a mouse air pouch model. a Assessment of membrane thickness (H&E staining) 6 and 24 h after crystals injection (1mg/ml) in C57BL/6 mice (N = 3–5/group). b Assessment of membrane thickness 6 and 24 h after crystals injection (1mg/ml) in BALB/c mice (N = 4–5/group). c Representative images of H&E and Iba1 staining 6 h after the injection for each group in C57BL/6 mice. d Quantification of the Iba1+ macrophage infiltration 6 h after the injection in C57BL/6 mice (N = 3–5/group). Results are expressed as Iba1+ stained surface (%) as assessed using the ImageJ software. e IL-1β gene expression in the air pouch membranes 6 h after the injection of crystals (1 or 2 mg/ml as indicated) (N = 5/group). Gene expression is expressed in relative expression (2^−ΔCt) with the HPRT gene used as a reference. sHA, synthetic apatite; CAL, human calcification; H&E, hematoxylin and eosin; C, crystals.*p < 0.05