Fig. 2

Autophagy is involved in the cytokine production of DCs. DCs from normal controls (n=8) and active BD patients (n=8) were assessed. a–c DCs were stimulated with LPS (100ng/ml) for 24 h. Cytokines including TNF-α, IL-1β, and IL-6 were measured in the culture supernatants by ELISA. d–i DCs from the normal controls were treated with 3-MA (10mM) or rapamycin (100nM) together with LPS (100ng/ml) for 24 h. TNF-α, IL-1β, and IL-6 were measured in the culture supernatants by ELISA. j–o DCs from active BD patients were treated with 3-MA (10mM) or rapamycin (100nM) together with LPS (100ng/ml) for 24 h. TNF-α, IL-1β, and IL-6 were measured in the culture supernatants by ELISA. *p<0.05, **p<0.01