Fig. 3

MiR-155 is involved in the cytokine production of DCs induced by dysfunctional autophagy. DCs from normal controls (n=8) and active BD patients (n=8) were assessed. DCs were transfected with control mimics and miR-155 mimics at a final concentration of 100 nM. After 48 h, DCs were stimulated with 100ng/mL LPS for 24 h. a Expression levels of LC3-I, LC3-II, Beclin-1, and P62 protein in DCs from normal controls and active BD patients were quantified by Western blot. b–d Quantification of expression levels of LC3-II, Beclin-1, and P62 proteins. e–g TNF-α, IL-1β, and IL-6 were measured in the culture supernatants by ELISA. *p<0.05, **p<0.01