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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: The heterogeneous human memory CCR6+ T helper-17 populations differ in T-bet and cytokine expression but all activate synovial fibroblasts in an IFNγ-independent manner

Fig. 1

CCR6+ Th memory subpopulations differ in cytokine production, but not in RORC expression. a Gating strategy for the four subpopulations. b Gene transcription levels of sorted cells, directly ex vivo (upper panel) or after 3 days of stimulation with anti-CD3 and anti-CD28 (lower panel) is determined using RT-PCR. c Representative flow cytometry plot of IL-17A, IL-22, and IFNγ expression after 3 days of stimulation from one healthy donor. d Production of IL-17A, IL-22, IFNγ, and GM-CSF after 3 days of stimulation with anti-CD3 and anti-CD28 as measured by ELISA. Data represent mean ± SEM of n = 3–8 healthy controls of at least 2 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Arthritis Research & Therapy

ISSN: 1478-6362

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