Fig. 1

High level of circ-BRWD1 in OA cartilage tissues and IL-1β-induced CHON-001 cells. A QRT-PCR assay was conducted for circ-BRWD1 level in OA and non-OA cartilage tissues. B QRT-PCR assay was employed to determine circ-BRWD1 level in different doses of IL-1β-treated CHON-001 cells. C QRT-PCR assay was used for the expression of circ-BRWD1 in the nuclear and cytosolic fractions of CHON-001 cells. D QRT-PCR assay was used to determine the expression levels of circ-BRWD1 and linear BRWD1 in CHON-001 cells treated with or without RNase R. The expression of circ-BRWD1 and BRWD1 was examined by 2^-ΔΔCt method with normalization to GAPDH. **P < 0.001, ****P < 0.0001