Fig. 2

Circ-BRWD1 level was increased in IL-1β-treated CHON-001 cells-derived exosomes. A The morphology of isolated particles was analyzed using TEM. B The protein levels of CD9 and CD63 were measured by western blot assay. C The level of circ-BRWD1 in the exosomes derived from IL-1β-treated CHON-001 cells was examined by qRT-PCR assay. D Exosomes derived from IL-1β-treated CHON-001 cells were added into CHON-001 cells and then circ-BRWD1 level in CHON-001 cells was determined by qRT-PCR assay. E After IL-1β-treated CHON-001 cells were treated with or without GW4869 for 2 h and then exposed to IL-1β, the expression level of circ-BRWD1 was determined by qRT-PCR assay. The expression of circ-BRWD1 was examined by 2^-ΔΔCt method with normalization to GAPDH. **P < 0.01, ***P < 0.001, ****P < 0.0001