Fig. 5

Circ-BRWD1 silencing promoted cell viability and inhibited apoptosis, inflammation and ECM degradation in IL-1β-stimulated CHON-001 cells by binding to miR-1277. CHON-001 cells were assigned to control, IL-1β, IL-1β+si-NC, IL-1β+si-circ-BRWD1, IL-1β+si-circ-BRWD1+anti-miR-NC, and IL-1β+si-circ-BRWD1+anti-miR-1277 groups. A QRT-PCR assay was adopted for miR-1277 level in CHON-001 cells. B–D The viability and apoptosis of CHON-001 cells were evaluated by CCK-8 assay and flow cytometry analysis, respectively. E The proliferation of CHON-001 cells was assessed by EDU assay. F The protein levels of CyclinD1 and Bax were measured by western blot assay. G ELISA was conducted for the levels of IL-6 and IL-8 in CHON-001 cells. H Western blot assay was carried out for the protein levels of MMP13 and aggrecan in CHON-001 cells. The expression of miR-1277 was examined by 2^-ΔΔCt method with normalization to U6. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001