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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Differential impacts of TNFα inhibitors on the transcriptome of Th cells

Fig. 1

Differential impacts of TNFis on the expression of Th cytokines. AE PBMCs from healthy donors were stimulated with anti-CD3 and anti-CD28 for 24 h in the absence or presence of indicated drugs at indicated concentration. In A, 1X equals 25 μg/ml for ada, 10 μg/ml for eta, 10 μg/ml for cert, and 20 μg/ml for toc. 5X was used for all drugs in BE. The transcript levels of the indicated genes in the PBMCs were measured with qPCR (AC, E), and the protein levels of IL17F and IL17A in the supernatant were quantified with ELISA (D). The levels of anti-CD3-stimulated samples were arbitrarily set as 1. In D, the level of 1 ranges from 26 to 137 pg/ml for IL-17F and 66-343 pg/ml for IL-17A. F. Human synovial fibroblasts were stimulated with TNFα (10 ng/ml) for 6 h in the presence or absence of the indicated TNFis at 5X concentration. The transcript level of IL-6 was measured with qPCR (N = 3)

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