Fig. 5
From: Differential impacts of TNFα inhibitors on the transcriptome of Th cells
Non-Th cell-dependent and independent mechanisms of ada. A Th cells were first purified from resting PBMCs then stimulated in the presence of absence of ada (5X). The transcript levels of indicated genes were measured with qPCR. The levels of anti-CD3-stimulated samples were arbitrarily set as 1. B–E Th cells in the context of PBMCs, separated from non-Th cells in transwells, or without non-Th cells were stimulated in the absence or presence of ada (5X) for 24 h. A schematic diagram of the experimental design is illustrated in B. Th cells were then purified and their expression of cytokine genes (C), type 1 IFN-inducible genes (D), as well as CD96 and SIRPG (E) was examined with qPCR