Fig. 6
From: Differential impacts of TNFα inhibitors on the transcriptome of Th cells
Dependence of CD14+ cells and Fc-mediated interaction for the effect of ada. A–C PBMCs were depleted of indicated populations through negative selection. The depletion was confirmed with FACS. Representative FACS plots are shown in A. The depleted PBMCs were stimulated with anti-CD3/anti-CD28 in the presence or absence of ada (5X). The transcript levels of indicated cytokines in the stimulated PBMCs were quantified with qPCR (B). Supernatant from three of the experiments was also subjected to IL-2 ELISA (C). D PBMCs were pre-treated with control human IgG1 (100 μg/ml) for 30 min, and then stimulated with anti-CD3/anti-CD28 in the presence or absence of ada (5X) or eta (5X) for 24 h. The transcript levels of indicated cytokines in PBMCs were measured with qPCR. The levels of samples stimulated in the absence of IgG1 or TNFi were arbitrarily set as 1