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Table 2 Components of TSG6-mediated HC-HA crosslinking reaction mixtures

From: Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation

Reaction HA 50 kDa (10 mg/mL) IαI (877 μg/mL) TSG6 (300 μg/mL) MgCl2 (125 mM) DPBS
HA 4.5 μL 0 μL 0 μL 1.0 μL 119.5 μL
HA + IαI 4.5 μL 37.0 μL 0 μL 1.0 μL 82.5 μL
HA + TSG6 4.5 μL 0 μL 33.0 μL 1.0 μL 86.5 μL
HA + IαI + TSG6 4.5 μL 37.0 μL 33.0 μL 1.0 μL 49.5 μL