Table 2 Components of TSG6-mediated HC-HA crosslinking reaction mixtures
Reaction | HA 50 kDa (10 mg/mL) | IαI (877 μg/mL) | TSG6 (300 μg/mL) | MgCl2 (125 mM) | DPBS |
|---|---|---|---|---|---|
HA | 4.5 μL | 0 μL | 0 μL | 1.0 μL | 119.5 μL |
HA + IαI | 4.5 μL | 37.0 μL | 0 μL | 1.0 μL | 82.5 μL |
HA + TSG6 | 4.5 μL | 0 μL | 33.0 μL | 1.0 μL | 86.5 μL |
HA + IαI + TSG6 | 4.5 μL | 37.0 μL | 33.0 μL | 1.0 μL | 49.5 μL |