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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Incidence and severity of G6PI-induced arthritis are not increased in genetically distinct mouse strains upon aging

Fig. 2

Upon arthritis FLS from old mice are more senescent and show higher glycolysis. Young and old mice were immunized with G6PI. Before or 56 days after immunization, synovial cells were extracted from the small joints and cultured. a, b At passage P4 FLS metabolism was analyzed. Basal OCR (a left) or ECAR (b left) were calculated as ratio of the values obtained for FLS from old mice vs FLS from young mice at d0 (average age: young: 13 weeks, n=3, old: 108 weeks, n=3) or d56 (average age young: 32 weeks, n=4, old: 89 weeks, n=4). Metabolic curves were normalized to the basal OCR (a right) or basal ECAR (b right) before addition of FCCP and oligomycin (1 μM each), and the induced maximal metabolic capacities are summarized in the diagrams. c FLS extracted 56 days after immunization were tested at P4 in a collagen matrix invasion assay. After 36 h, the numbers of migrated cells were analyzed. Representative results are shown in the left panel. Experiments are summarized in the diagram (average age: young: 16 weeks, old: 96 weeks, n=3). d At passage P4, FLS extracted 56 days after immunization were tested in a senescence assay and the percentage of senescent cells (defined as βGalactosidase-positive) was analyzed. Representative results are shown in the left panel. Data are summarized in the diagram (average age: young: 16 weeks, old: 95 weeks, n=2). Statistical testing was performed as described in the “Methods” section. n.s. non-significant

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