Fig. 1

Histologic analysis of the salivary glands of 6-week-old LAT mice and of the littermates. A H&E staining and M&T staining of salivary glands. The boxed areas in the upper panels are shown in detail in the lower panels. Scale bars, 200 μm. Representative images of 3 mice per group. H&E, hematoxylin and eosin stain; M&T, Masson’s trichrome stain. B Focus score of salivary glands. Histologic evaluation was performed in a blinded manner (littermate, n = 3; LAT mice, n = 3). C Fibrosis score of salivary glands. Histologic evaluation was performed in a blinded manner (littermate, n = 3; LAT mice, n = 3). D Images are representative of salivary gland slices of LAT mice stained for CD4 (green), B220 (red), and 4′6-diamino-2-phenylindole (DAPI) (blue). Top: low magnification images; second row from the top: the part focused on CD4⁺ cells; third row from the top: the part focused on B220⁺ cells; bottom row: images stained for isotype control. Scale bars (top, 50 μm; second and third rows from the top, 10 μm; bottom row, 50 μm). E Salivary gland slices of LAT mice stained for CD138 or isotype control. Scale bar,100 μm. *p < 0.05, unpaired t test