Fig. 2

Upregulation of Th2 cytokines and the CCL8–CCR8 axis in 6-week-old LAT mice. A Il4 mRNA levels in spleens, cervical lymph nodes (cLNs), and salivary glands (SGs) were determined by qRT-PCR. The expression levels were calculated as relative amounts normalized to Gapdh (littermate, n = 3; LAT, n = 3). B Il10 mRNA levels in spleens, cLNs, and SGs were determined by qRT-PCR. The expression levels were calculated as relative amounts normalized to Gapdh (littermate, n = 3; LAT, n = 3). C Ccl8 mRNA levels in each tissue were determined by means of qRT-PCR. The expression levels were calculated as relative amounts normalized to Gapdh (littermate, n = 3; LAT, n = 3). D Ccr8 mRNA levels in each tissue was determined by means of qRT-PCR. The expression levels were calculated as relative amounts normalized to Gapdh (littermate, n = 3; LAT, n = 3). E Serum CCL8 levels were determined by ELISA (littermate, n = 4; LAT, n = 5). F Splenocytes from littermates and LAT mice were assessed by flow cytometry for cytokine production after stimulation with phorbol myristate acetate and ionomycin for 4 h. The proportion of CD4⁺ cells that producing each cytokine was quantified (littermate, n = 3; LAT, n = 3). G Splenocytes and mononuclear cells infiltrated in SGs from LAT mice were assessed by flow cytometry for cytokine production after stimulation with phorbol myristate acetate and ionomycin for 4 h. The proportion of CD4⁺ cells that producing each cytokine was quantified. *p < 0.05, Unpaired t test. The data are presented as means ± SEMs