Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: S100A8/A9 is not essential for the development of inflammation and joint pathology in interleukin-1 receptor antagonist knockout mice

Fig. 3

The absence of S100A9 affects the composition of myeloid populations in the spleen of Il1rn−/− mice. Total cell counts in the bone marrow were significantly higher in mice deficient in Il1rn, but not different between Il1rn−/− and Il1rn−/−XS100a9−/− mice. Flow cytometry analysis showed that the number of CD11b+Ly6Ghigh neutrophils, pro-inflammatory CD11b+Ly6GLy6Chigh monocytes and the more immunomodulatory CD11b+Ly6GLy6Clow monocytes, and CD11bneg/lowLy6GLy6Chigh osteoclast progenitors were significantly increased in the absence of Il1rn. However, no significant differences were observed for any of these populations between Il1rn−/−XS100a9−/− and Il1rn−/− mice (A). In contrast to the bone marrow, total cell numbers were not increased in the spleen of Il1rn-deficient mouse strains. Additionally, an interaction between the absence of Il1rn and S100a9 was present for the numbers of neutrophils and Ly6Chigh monocytes but not for the Ly6Clow monocytes (B). Scatterplots are shown, with horizontal and vertical lines representing mean ± SEM values. *P < 0.05, **P < 0.01, ***P < 0.001. Results of the two-way ANOVA with interaction are presented in the table

Back to article page