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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Cross-reactivity of IgM anti-modified protein antibodies in rheumatoid arthritis despite limited mutational load

Fig. 2

Cross-reactivity of monoclonal AMPA-IgM. A ELISA with 10 μg/ml mAb on different citrullinated peptides and arginine-control peptides for 2D5 and 1G8 or with different acetylated peptides and lysine-control peptides for 1E3 (coated 10 μg/ml antigen). The cut-off for positivity was based on the anti-TT IgM control mAb. B Titration ELISA of the mAbs 2D5, 1G8 and 1E3 on PTM-Cyclic Peptide 2 (coated 1 μg/ml antigen). C Heatmap of a PTM antigen ELISA with 10 μg/ml monoclonal AMPA-IgMs 2D5, 1G8 and 1E3 and anti-TT-IgM control mAb on different peptides (coated 10 μg/ml antigen) and D proteins (coated 10 μg/ml antigen). Binding is represented by the optical density at 415 nm. E Western blot of monoclonal AMPA-IgMs 2D5, 1G8 and 1E3 (2 μg/ml) on PTM-Fibrinogen and unmodified fibrinogen (0.25–2 μg)

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