Fig. 4

Antibody valency: AMPA-IgM expressed as IgG. A Analytical size exclusion chromatography of pentameric IgM and monomeric IgG mAb. 30 µg mAb were loaded on a Superose6Increase 10/30 GL column. B ELISA with 2D5 and 1G8 as IgM and as IgG (10 μg/ml) on different citrullinated peptides and arginine-control peptides (coated 10 μg/ml antigen) and C citrullinated and unmodified proteins (coated 10 μg/ml antigen). D ELISA with 1E3 as IgM and as IgG (10 μg/ml) on different acetylated peptides and lysine-control peptides (coated 10 μg/ml antigen) and E acetylated and unmodified proteins (coated 10 μg/ml antigen). All experiments were replicated 2–3 times with similar outcomes. Binding is represented by the optical density at 415 nm