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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Cross-reactivity of IgM anti-modified protein antibodies in rheumatoid arthritis despite limited mutational load

Fig. 5

Complement activation of AMPA IgM. A Complement C3c deposition ELISA of the mAb TT-IgM and 2D5-IgM on a CCP2 (1 μg/ml) or CHcitP2 (1 μg/ml) coated plate. B C3c desposition ELISA with 2D5-IgM and and 2D5 GL with and without CDR3 mAb bound to Cit-vimentin 59-74 (coated 10 μg/ml antigen). C CCP2 titration ELISA of 2D5-IgM, 2D5-IgG and 2G9-IgG mAbs using equal molarities (coated 1 μg/ml CCP2). D C3c deposition ELISA with 2D5-IgM, 2D5-IgG and 2G9-IgG mAbs using equal molarities (coated 1 μg/ml CCP2). All experiments were replicated 2–3 times with similar outcomes

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