Table 1 Characteristics of the single cell sorted AMPA-IgM expressing B cell sequences. IGHVDJ, IGLVJ and number of IGHV mutations were determined by alligment to the IMGT database
| Â | 2D5 | 1G8 | 1E3 | Anti-TT |
|---|---|---|---|---|
Antigen for isolation | CCP2 | CCP2 | Acetylated-vimentin (HC55) | Tetanus Toxoid |
Patient | 5 | 5 | 6 | 7 |
Heavy chain | μ | μ | μ | μ |
IGHV | V7-4-1 | V7-4-1 | V4-59 | V5-10-1 |
IGHD | D4-17 | D7-27 | D5-24 | D3-10 |
IGHJ | J6 | J4 | J6 | J3 |
IGH-CD3 aa | CARGTHDYADYYYYGMDVW | CARGTEDGDAYFDYW | CARHIQSLVTTGYYTYPMDVW | CARSYYGPGNYDAFDIW |
#IGHV mutations nt | 3 | 7 | 16 | 16 |
Light chain | λ | λ | κ | κ |
IGLV | V10-54 | V4-69 | V2-28 | V1-9 |
IGLJ | J3 | J2 | J2 | J4 |
IGL-CDR3aa | CSAWDNSLSAWVF | CQTWGTDTVVF | CMQALQNPRAF | CQQLHSYPRTF |
#IGLV mutations nt | 4 | 8 | 7 | 9 |