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Fig. 4 | Arthritis Research & Therapy

Fig. 4

From: Isopsoralen ameliorates rheumatoid arthritis by targeting MIF

Fig. 4

Identification of the targets of IPRN. a GO analysis of the potential targets of IPRN identified by network pharmacology analysis. b Molecular docking analysis of IPRN and the MIF protein (PDB ID: 1GCZ). c Recombinant MIF was incubated with l-dopachrome methyl ester substrate with or without IPRN (10 or 50 μmol/L) or ISO-1 (50 μmol/L). The tautomerase activity was assessed by measuring the absorbance at 475 nm over 6 min. d Western blotting analysis of MIF protein expression in RA FLSs after incubation with IPRN. GAPDH was used as a loading control to normalize MIF expression. e CETSA assay of the stability of the MIF protein at 54 °C and 57 °C in the absence or presence of IPRN. The values are expressed as the mean ± SD of at least three independent experiments. **P< 0.01 and ***P < 0.001

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