Skip to main content
Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Isopsoralen ameliorates rheumatoid arthritis by targeting MIF

Fig. 5

IPRN inhibits inflammation in a MIF-dependent way. a In the presence of IPRN (10 μM or 20 μM), RA FLSs were treated hMIF (5 ng/mL) or not for 24 h. Total RNA was extracted and the expression of IL-6, IL-8, MMP3, and CXCL9 at mRNA levels were investigated by RT-qPCR. b, c RA FLSs transfected with control (siCtrl) or MIF siRNA (siMIF) were stimulated with TNF-α (10 ng/mL). The mRNA expression of IL-6, IL-8, MMP3, and CXCL9 was tested by RT-qPCR (b). Supernatants of RA FLSs were collected and used as conditioned medium to culture HUVECs to perform tube formation assays (c). The values are expressed as the mean ± SD (n=6) and are from three independent experiments with similar results. *P < 0.05, **P < 0.01, and ***P< 0.001

Back to article page