Fig. 2

Co-expression profiling of axSpA signature probe-sets in reference transcriptomes. Co-expression profiling of up- and downregulated axSpA signature probe-sets of microarray analysis of cohort 1 (A + B) and axSpA signature genes of RNA-seq analysis of cohort 2 matched to microarray probe-sets (C + D) in reference transcriptomes. Reference transcriptomes comprised transcriptomes from (1) bone marrow (including hematopoietic stem cells (HSC), early and late myeloid progenitors, and polymorph nuclear neutrophils (PMN)), (2) blood cells (comprising BDCA1⁺ and BDCA3⁺ dendritic cells (DCs), CD15⁺ neutrophils, CD16⁻, and CD16⁺ monocytes), (3) blood leukocytes before and after G-CSF treatment, and (4) monocytes stimulated in vitro with or without the indicated cytokines. Up- or downregulated signature probe-sets lists were applied to the reference transcriptomes. After quantile normalization, the relative signal intensities from the reference transcriptomes were correlated and obtained correlation coefficients were hierarchically clustered as shown by the co-expression matrixes. This probe-set order was applied to display relative probe-set intensities of the 70 reference transcriptomes. Red indicated increased signal expression (max = 2) or positive correlation (max = 1) and blue indicates decreased signal expression (min = − 2) or negative correlation (min = − 1). Clusters of co-expression are boxed and examples of genes within individual clusters are given on the right