Fig. 6

DNA methylation downregulated the expression of ACSM5 in a DNMT1-dependent manner. A Pictures showing the morphology of isolated LFCs with 1st and 3rd passage. B Identification of the phenotype of cultured ligamentum flavum (LF) cells using immunofluorescence staining. Immunofluorescence staining for collagen I and Vimentin in cultured cells; n = 3 experiments. C The relative expression level of COL4A3, HSPA6, PIGC, and ACSM5 compared between LFCs from the HLF group and LFCs from the non-HLF group. D The relative DNA methylation level of ACSM5 compared between LFCs from HLF group and LFCs from non-HLF group. E The relative DNA methylation level of ACSM5 in LFCs from HLF patients with the treatment of different concentrations of 5-AzaC. F qPCR and Western Blot results showing the expression level of ACSM5 in LFCs from HLF patients with the treatment of different concentrations of 5-AzaC. G The relative expression level of DNMTs in LFCs from HLF patients with the treatment of indicated DNMT’s shRNA. H qPCR and Western Blot results showing the expression level of ACSM5 in LFCs from HLF patients with the treatment of indicated DNMT’s shRNA. Data were presented as the mean ± SME of three independent experiments. ^N.S.P > 0.05, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001