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Fig. 8 | Arthritis Research & Therapy

Fig. 8

From: Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy

Fig. 8

DNMT1 regulates the proliferation, fibrosis, and apoptosis of LFCs via downregulating ACSM5 expression. A The relative mRNA and protein expression level of ACSM5 in LFCs from HLF patients with the treatment of indicated shRNAs. B CK8 assays of LFCs from HLF patients with the treatment of indicated shRNAs. Absorbances were measured at 24 h, 48 h, 72 h, and 96 h post cell seeding. C The relative caspase3 activity of LFCs from HLF patients with the treatment of indicated shRNAs. D Flow graph and statistical results of apoptosis assays using LFCs from HLF patients with the treatment of indicated shRNAs. E RT-PCR results showing the expression level of COL1, COL3, MMP13, and MMP2 in LFCs from HLF patients with the treatment of indicated shRNAs. F Western Blot results showing the expression level of COL1, COL3, MMP13, and MMP2 in LFCs from HLF patients with the treatment of indicated shRNAs. Data were presented as the mean ± SME of three independent experiments. Tukey’s test, #P < 0.05, ##P < 0.01, ###P < 0.001. Dollar sign ($) symbol represented the significance compared to the sh-ACSM5 group, $P < 0.05, $$P < 0.01, $$$P < 0.001. Ampersand (&) symbol represented the significance compared to the shDNMT1 group, &P < 0.05, &&P < 0.01, &&&P < 0.001

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