Table 4 The primer sequences of RT-PCR and the sequences of shRNAs
Gene | Sequences (5′-3′) |
|---|---|
ACSM5 | Forward primer: ACACTGGCTGGGTGAAGG Reverse primer : ACAGCAGAGGGTGGTTATCG |
COL4A3 | Forward primer: AGCAAGGGTTGTGTCTGTAAAG Reverse primer :CAGAAAATCCTGGCAATCCACT |
HSPA6 | Forward primer: CAGTCAGATCATCTTCTCGA Reverse primer :CTTCCATGAAGTGGTTCACGA |
MACC1 | Forward primer: CATTTTCGGTCAGGAAGAATTGC Reverse primer :TGGAAGCATTATTACCACGAAGG |
PIGC | Forward primer: GCCCCGAAGCGGGAAAAAG Reverse primer :CAACACAGCCAAGTTCCCGA |
GAPDH | Forward primer: AAGTATGACAACAGCCTCAAG Reverse primer :TCCACGATACCAAAGTTGTC |
DNMT1 shRNA | CCGGGACGACCCTGACCTCAAATATCTCGAGATATTTGAGGTCAGGGTCGTCTTTTTG |
DNMT3A shRNA | CCGGCCCAAGGTCAAGGAGATTATTCTCGAGAATAATCTCCTTGACCTTGGGTTTTTG |
DNMT3B shRNA | CCGGAGTGCCGACAGCTCTCCAATACTCGAGTATTGGAGAGCTGTCGGCACTTTTTTTG |
ACSM5 shRNA | CCGGGATGTGCAGATTGTGGATGATCTCGAGATCATCCACAATCTGCACATCTTTTTTG |