Fig. 5

Monitoring DEX-associated treatment response using LS301. Representative fluorescence images of arthritic mice imaged with LS301 before (left) and after (right) dexamethasone (DEX) treatment. C57BL/6 mice with induced arthritis (STA) (n = 3 per group) were imaged with 6 nmol intravenous LS301 at day 3 post-disease induction. Mice then A remained untreated as controls or B were treated with daily doses of intraperitoneal dexamethasone (10 mg/kg/dose) over a 6-day period. At day 9 post-disease induction, mice were imaged again with 6 nmol intravenous LS301. Whole body near-infrared fluorescence images were taken on the Pearl animal imaging system with λ = 820 nm. Images were taken at 18h post-injection with mice in dorsal orientation. Numbers denote individual clinical paw scores at the time of imaging. C Comparison of average total extremity fluorescence (quantitated from ROIs) per mouse described in A and B. C Total paw arthritis scores and change in ankle thickness from baseline of mice described in A and B