Fig. 3

The TSP1 domain of CTGF is the direct region that interact with ANXA2. A Schematic representations of full-length CTGF and its deletion mutants. B Co-IP using anti-ANXA2 and anti-FLAG antibodies with lysates of various deletion mutants transfected into HEK293T cells. C Fluorescence expression of pBiFC-VN155 (I152L)-ANXA2 co-transfected with pBiFC-VC155-CTGF or pBiFC-VC155-CTGF-∆TSP1 in HEK293T cells at a magnification of × 200. D Structure of TSP1 domain of CTGF by homology modeling. E Predicted binding mode of ANXA2/CTGF-TSP1, green and blue represent the ANXA2 and CTGF-TSP1 domain regions, respectively. F Root mean square deviation (RMSD) of the backbone atoms (C, CA, and N) of the ANXA2/CTGF-TSP1 complex as a function of time. G Conformations of the complex during molecular dynamics simulation