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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Sustained microglial activation in the area postrema of collagen-induced arthritis mice

Fig. 3

Morphological classification of microglia and increase of activated microglia during arthritis. A Morphological plots of each cell (2118 microglia cells from 51 mice) on the first two principal component (PC-1 and PC-2) coordinate planes with examples of their morphology (PID 21: CIA, n = 6, saline, n = 6; PID 35: CIA, n = 7, saline, n = 10; PID 56: CIA = 6, saline, n = 6; and PID 84: CIA, n = 6, saline, n = 4). Microglia were classified into cluster-1 and cluster-2 by hierarchical clustering analysis. Gray dashed lines reflect loading plots. B Proportion of cluster-1 microglia during each disease phase. There were no differences between groups on PID 21 by chi-squared test. Conversely, the proportion in the collagen-induced arthritis (CIA) group was higher than the saline groups on PID 35, 56, and 84 by chi-squared test. Abbreviations: AR, aspect ratio; Feret, Feret diameter; Peri, perimeter; Peri/Area, ratio of perimeter to area; PID, post-immunization day; Min Feret, minimum Feret diameter; Minor, minor diameter; Major, major diameter; Round, roundness

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