Table 1 Antibodies and dyes used for the broad spectrum immune cell panel
From: Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries
Broad spectrum panel | ||||
|---|---|---|---|---|
Marker | Clone | Fluorophore | Dilution | Vendor |
Zombie viability dye | N/A | nIR | 1:100 | Biolegend (San Diego, CA) |
CD3 | SK7 | AlexaFluor 700 | 1:10 | Biolegend |
CD14 | M5E2 | PB | 1:10 | BD Biosciences (San Jose, CA) |
CD16 | 3G8 | APC | 1:10 | Biolegend |
CD19 | HIB19 | PE-Cy5 | 1:10 | Biolegend |
CD45 | HI30 | PE | 1:10 | Sony (San Jose, CA) |
CD86 | FUN-1 | BV510 | 1:20 | BD Biosciences |
HLA-DR | L243 | BV605 | 1:20 | Biolegend |