From: Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries
T cell panel | ||||
---|---|---|---|---|
Marker | Clone | Fluorophore | Dilution | Vendor |
Zombie viability dye | N/A | Aqua | 1:100 | Biolegend (San Diego, CA) |
CD3 | SK7 | AlexaFluor 700 | 1:20 | Biolegend |
CD4 | SK3 | BUV805 | 1:20 | BD Biosciences (San Jose, CA) |
CD8 | SK1 | APC-Cy7 | 1:10 | Biolegend |
CD14 | MOP9 | BUV395 | 1:20 | BD Biosciences |
CD25 | 2A3 | BB515 | 1:40 | BD Biosciences |
CD38 | HIT2 | BV421 | 1:20 | Biolegend |
CD45 | HI30 | PE-Cy5 | 1:20 | Biolegend |
CD45RA | HI100 | PerCP-Cy5.5 | 1:10 | Biolegend |
CD127 | A019D5 | BV650 | 1:20 | Biolegend |
CCR4 | L291H4 | BV605 | 1:20 | Biolegend |
CCR6 | G034E3 | BV785 | 1:20 | Biolegend |
CCR7 | 150503 | PE-CF594 | 1:20 | BD Biosciences |
CCR10 | 314305 | APC | 1:20 | R&D Systems (Minneapolis, MN) |
CXCR3 | 1C6/CXCR3 | PE | 1:2.5 | BD Biosciences |
CXCR5 | J252D4 | PE-Cy7 | 1:78 | Biolegend |
HLA-DR | G46-6 | BUV661 | 1:313 | BD Biosciences |