Skip to main content

Table 2 Antibodies and dyes used for the T cell panel

From: Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries

T cell panel
Marker Clone Fluorophore Dilution Vendor
Zombie viability dye N/A Aqua 1:100 Biolegend (San Diego, CA)
CD3 SK7 AlexaFluor 700 1:20 Biolegend
CD4 SK3 BUV805 1:20 BD Biosciences (San Jose, CA)
CD8 SK1 APC-Cy7 1:10 Biolegend
CD14 MOP9 BUV395 1:20 BD Biosciences
CD25 2A3 BB515 1:40 BD Biosciences
CD38 HIT2 BV421 1:20 Biolegend
CD45 HI30 PE-Cy5 1:20 Biolegend
CD45RA HI100 PerCP-Cy5.5 1:10 Biolegend
CD127 A019D5 BV650 1:20 Biolegend
CCR4 L291H4 BV605 1:20 Biolegend
CCR6 G034E3 BV785 1:20 Biolegend
CCR7 150503 PE-CF594 1:20 BD Biosciences
CCR10 314305 APC 1:20 R&D Systems (Minneapolis, MN)
CXCR3 1C6/CXCR3 PE 1:2.5 BD Biosciences
CXCR5 J252D4 PE-Cy7 1:78 Biolegend
HLA-DR G46-6 BUV661 1:313 BD Biosciences