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Table 2 Antibodies and dyes used for the T cell panel

From: Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries

T cell panel

Marker

Clone

Fluorophore

Dilution

Vendor

Zombie viability dye

N/A

Aqua

1:100

Biolegend (San Diego, CA)

CD3

SK7

AlexaFluor 700

1:20

Biolegend

CD4

SK3

BUV805

1:20

BD Biosciences (San Jose, CA)

CD8

SK1

APC-Cy7

1:10

Biolegend

CD14

MOP9

BUV395

1:20

BD Biosciences

CD25

2A3

BB515

1:40

BD Biosciences

CD38

HIT2

BV421

1:20

Biolegend

CD45

HI30

PE-Cy5

1:20

Biolegend

CD45RA

HI100

PerCP-Cy5.5

1:10

Biolegend

CD127

A019D5

BV650

1:20

Biolegend

CCR4

L291H4

BV605

1:20

Biolegend

CCR6

G034E3

BV785

1:20

Biolegend

CCR7

150503

PE-CF594

1:20

BD Biosciences

CCR10

314305

APC

1:20

R&D Systems (Minneapolis, MN)

CXCR3

1C6/CXCR3

PE

1:2.5

BD Biosciences

CXCR5

J252D4

PE-Cy7

1:78

Biolegend

HLA-DR

G46-6

BUV661

1:313

BD Biosciences